Jamming transition of a granular pile below the angle of repose

We study experimentally the relaxation towards mechanical equilibrium of a granular pile which has just experienced an avalanche and discuss it in the more general context of the granular jamming transition. Two coexisting dynamics are observed in the surface layer: a short time exponential decay consisting in rapid and independent moves of grains and intermittent bursts consisting in spatially correlated moves lasting for longer time. The competition of both dynamics results in long-lived intermittent transients, the total duration of which can late more than a thousand of seconds. We measure a two-time relaxation function, and relate it via a simple statistical model to a more usual two-time correlation function which exhibits strong similarities with auto-correlation functions found in aging systems. Localized perturbation experiments also allow us to test the pile surface layer receptivity.Comment: 9 pages, 10 figure

Flow rule, self-channelization and levees in unconfined granular flows

Unconfined granular flows along an inclined plane are investigated experimentally. During a long transient, the flow gets confined by quasistatic banks but still spreads laterally towards a well-defined asymptotic state following a nontrivial process. Far enough from the banks a scaling for the depth averaged velocity is obtained, which extends the one obtained for homogeneous steady flows. Close to jamming it exhibits a crossover towards a nonlocal rheology. We show that the levees, commonly observed along the sides of the deposit upon interruption of the flow, disappear for long flow durations. We demonstrate that the morphology of the deposit builds up during the flow, in the form of an underlying static layer, which can be deduced from surface velocity profiles, by imposing the same flow rule everywhere in the flow.Comment: 4 pages, 5 figure

Ice-stream flow switching by up-ice propagation of instabilities along glacial marginal troughs

Ice-stream networks constitute the arteries of ice sheets through which large volumes of glacial ice are rapidly delivered from the continent to the ocean. Modifications in ice-stream networks have a major impact on ice sheet mass balance and global sea level. Reorganizations in the drainage network of ice streams have been reported in both modern and paleo-ice sheets and usually result in ice streams switching their trajectory and/or shutting down. While some hypotheses for the reorganization of ice streams have been proposed, the mechanisms that control the switching of ice streams remain poorly understood and documented. Here, we interpret a flow switch in an ice-stream system that occurred prior to the last glaciation on the northeastern Baffin Island shelf (Arctic Canada) through glacial erosion of a marginal trough, i.e., deep parallel-to-coast bedrock moats located up-ice of a cross-shelf trough. Shelf geomorphology imaged by high-resolution swath bathymetry and seismo-stratigraphic data in the area indicate the extension of ice streams from Scott and Hecla &amp; Griper troughs towards the interior of the Laurentide Ice Sheet. Up-ice propagation of ice streams through a marginal trough is interpreted to have led to the piracy of the neighboring ice catchment that in turn induced an adjacent ice-stream flow switch and shutdown. These results suggest that competition for ice discharge between the two ice streams, which implies piracy of ice drainage basins via marginal troughs, was the driving mechanism behind ice flow switching. In turn, the enlargement of the ice catchment by piracy increased the volume and discharge of Scott Ice Stream, allowing it to erode deeper and flow farther on the continental shelf. Similar trough systems observed on many other glaciated continental shelves may be the product of such competition for ice discharge between catchments.</p

Elevated hepatocyte growth factor levels in osteoarthritis osteoblasts contribute to their altered response to bone morphogenetic protein-2 and reduced mineralization capacity

PURPOSE: Clinical and in vitro studies suggest that subchondral bone sclerosis due to abnormal osteoblasts is involved in the progression of osteoarthritis (OA). Human osteoblasts isolated from sclerotic subchondral OA bone tissue show an altered phenotype, a decreased canonical Wnt/ß-catenin pathway, and a reduced mineralization in vitro as well as in vivo. These alterations were linked with an abnormal response to BMP-2. OA osteoblasts release factors such as the hepatocyte growth factor (HGF) that contribute to cartilage loss whereas chondrocytes do not express HGF. HGF can stimulate BMP-2 expression in human osteoblasts, however, the role of HGF and its effect in OA osteoblasts remains unknown. Here we investigated whether elevated endogenous HGF levels in OA osteoblasts are responsible for their altered response to BMP-2. METHODS: We prepared primary human subchondral osteoblasts using the sclerotic medial portion of the tibial plateaus of OA patients undergoing total knee arthroplasty, or from tibial plateaus of normal individuals obtained at autopsy. The expression of HGF was evaluated by qRT-PCR and the protein production by western blot analysis. HGF expression was reduced with siRNA technique whereas its activity was inhibited using the selective inhibitor PHA665752. Alkaline phosphatase activity (ALPase) and osteocalcin release were measured by substrate hydrolysis and EIA respectively. Canonical Wnt/β-catenin signaling (cWnt) was evaluated both by target gene expression using the TOPflash TCF/lef luciferase reporter assay and western blot analysis of β-catenin levels in response to Wnt3a stimulation. Mineralization in response to BMP-2 was evaluated by alizarin red staining. RESULTS: The expression of HGF was increased in OA osteoblasts compared to normal osteoblasts and was maintained during their in vitro differentiation. OA osteoblasts released more HGF than normal osteoblasts as assessed by western blot analysis. HGF stimulated the expression of TGF-β1. BMP-2 dose-dependently (1 to 100ng/ml) stimulated both ALPase and osteocalcin in normal osteoblasts whereas, it inhibited them in OA osteoblasts. HGF-siRNA treatments reversed this response in OA osteoblasts and restored the BMP-2 response. cWnt is reduced in OA osteoblasts compared to normal, and HGF-siRNA treatments increased cWnt in OA osteoblasts almost to normal. Smad1/5/8 phosphorylation in response to BMP-2, which is reduced in OA osteoblasts, was corrected when these cells were treated with PHA665752. The BMP-2-dependent mineralization of OA osteoblasts, which is also reduced compared to normal, was only partially restored by PHA665752 treatment whereas 28days treatment with HGF reduced the mineralization of normal osteoblasts. CONCLUSION: OA osteoblasts expressed more HGF than normal osteoblasts. Increased endogenous HGF production in OA osteoblasts stimulated the expression of TGF-β1 and reduced their response to BMP-2. Inhibiting HGF expression or HGF signaling restored the response to BMP-2 and Smad1/5/8 signaling. In addition, decreased HGF signaling partly corrects the abnormal mineralization of OA osteoblasts while increased HGF prevents the normal mineralization of normal osteoblasts. In summary, we hypothesize that sustained elevated HGF levels in OA osteoblasts drive their abnormal phenotype and is implicated in OA pathophysiology

Improved Resolution of Reef-Coral Endosymbiont (Symbiodinium) Species Diversity, Ecology, and Evolution through psbA Non-Coding Region Genotyping

Ribosomal DNA sequence data abounds from numerous studies on the dinoflagellate endosymbionts of corals, and yet the multi-copy nature and intragenomic variability of rRNA genes and spacers confound interpretations of symbiont diversity and ecology. Making consistent sense of extensive sequence variation in a meaningful ecological and evolutionary context would benefit from the application of additional genetic markers. Sequences of the non-coding region of the plastid psbA minicircle (psbAncr) were used to independently examine symbiont genotypic and species diversity found within and between colonies of Hawaiian reef corals in the genus Montipora. A single psbAncr haplotype was recovered in most samples through direct sequencing (∼80–90%) and members of the same internal transcribed spacer region 2 (ITS2) type were phylogenetically differentiated from other ITS2 types by substantial psbAncr sequence divergence. The repeated sequencing of bacterially-cloned fragments of psbAncr from samples and clonal cultures often recovered a single numerically common haplotype accompanied by rare, highly-similar, sequence variants. When sequence artifacts of cloning and intragenomic variation are factored out, these data indicate that most colonies harbored one dominant Symbiodinium genotype. The cloning and sequencing of ITS2 DNA amplified from these same samples recovered numerically abundant variants (that are diagnostic of distinct Symbiodinium lineages), but also generated a large amount of sequences comprising PCR/cloning artifacts combined with ancestral and/or rare variants that, if incorporated into phylogenetic reconstructions, confound how small sequence differences are interpreted. Finally, psbAncr sequence data from a broad sampling of Symbiodinium diversity obtained from various corals throughout the Indo-Pacific were concordant with ITS lineage membership (defined by denaturing gradient gel electrophoresis screening), yet exhibited substantially greater sequence divergence and revealed strong phylogeographic structure corresponding to major biogeographic provinces. The detailed genetic resolution provided by psbAncr data brings further clarity to the ecology, evolution, and systematics of symbiotic dinoflagellates

Slippery sliding on icy Iapetus

Enigmatically, some landslides flow farther than normal frictional resistance allows. Cassini images of Saturn’s icy moon Iapetus reveal a multitude of long-runout landslides that may have been enabled by flash heating along the sliding surface

Investigating Coral Bleaching in a Changing Climate: Our State of Understanding and Opportunities to Push the Field Forward

[First Paragraph] Coral reefs throughout the world are facing the consequences of large-scale changes in Earth’s climate. In particular, ocean warming is leading to frequent coral bleaching, which is threatening the long-term stability of coral reefs. Coral bleaching is a stress response that results in the disassociation of the mutualistic symbioses (i.e., dysbiosis) between corals and their endosymbiotic algae (Symbiodinium spp.). In the past two decades, there have been four substantial bleaching events, which have affected large geographic areas across the globe, including the worst recorded bleaching event on the Great Barrier Reef in 2016 (Berkelmans et al. 2004; Eakin et al. 2010; Stella et al. 2016). These large-scale bleaching events, in combination with many local-scale stressors, have contributed substantially to global declines in coral populations. In addition, bleaching may lead to compromised coral immunity, possibly resulting in additional mortality by a range of post-bleaching diseases (Maynard et al. 2015, Randall et al. 2014). Given their link to patterns of global-climate change and projections of increased warming in the coming decades, mass coral bleaching events are a key concern. In addition, current climate projections estimate that global bleaching is expected to occur annually by late this century, with more than 90% of reefs facing long-term degradation (Frieler et al. 2012). Furthermore, in locations such as the Caribbean, frequent thermal anomalies and consecutive annual bleaching events are expected to be common in less than 25 years (van Hooidonk et al. 2015). In fact, large-scale bleaching two years in a row was documented for the first time in 2014-2015 in Hawaii and in the Florida Keys. However, not all corals (and other symbiotic cnidarians) are equally susceptible to thermal stress, and some corals have been shown to recover from bleaching more quickly than others. Likewise, not all reefs are equally susceptible, and depending on local conditions, susceptibility can vary from one event to the next. Such variability in resilience could be a cornerstone to reef persistence over the coming century. However, the research needed to test this hypothesis remains to be performed

Stability of bedforms in laminar flows with free surface: from bars to ripples

International audienceThe present paper is devoted to the formation of sand patterns by laminar flows. It focuses on the rhomboid beach pattern, formed during the backswash. A recent bedload transport model, based on a moving-grains balance, is generalized in three dimensions for viscous flows. The water flow is modelled by the full incompressible Navier–Stokes equations with a free surface. A linear stability analysis then shows the simultaneous existence of two distinct instabilities, namely ripples and bars. The comparison of the bar instability characteristics with laboratory rhomboid patterns indicates that the latter could result from the nonlinear evolution of unstable bars. This result, together with the sensibility of the stability analysis with respect to the parameters of the transport law, suggests that the rhomboid pattern could help improving sediment transport models, so critical to geomorphologists

Transcriptional Response of Two Core Photosystem Genes in Symbiodinium spp. Exposed to Thermal Stress

Mutualistic symbioses between scleractinian corals and endosymbiotic dinoflagellates (Symbiodinium spp.) are the foundation of coral reef ecosystems. For many coral-algal symbioses, prolonged episodes of thermal stress damage the symbiont\u27s photosynthetic capability, resulting in its expulsion from the host. Despite the link between photosynthetic competency and symbiont expulsion, little is known about the effect of thermal stress on the expression of photosystem genes in Symbiodinium. This study used real-time PCR to monitor the transcript abundance of two important photosynthetic reaction center genes, psbA(encoding the D1 protein of photosystem II) and psaA (encoding the P700 protein of photosystem I), in four cultured isolates (representing ITS2-types A13, A20, B1, and F2) and two in hospite Symbiodinium spp. within the coral Pocillopora spp. (ITS2-types C1b-c and D1). Both cultured and in hospite Symbiodinium samples were exposed to elevated temperatures (32°C) over a 7-day period and examined for changes in photochemistry and transcript abundance. Symbiodinium A13 and C1b-c (both thermally sensitive) demonstrated significant declines in both psbA and psaA during the thermal stress treatment, whereas the transcript levels of the other Symbiodinium types remained stable. The downregulation of both core photosystem genes could be the result of several different physiological mechanisms, but may ultimately limit repair rates of photosynthetic proteins, rendering some Symbiodinium spp. especially susceptible to thermal stress